OriGene

POFUT2 Monoclonal Antibody (OTI3A12), TrueMAB™

Product Image Gallery

POFUT2 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Pof T2 (RC209505, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-Pof T. Positive lysates LY408775 (100 µg) and LC408775 (20 µg) can be purchased separately from OriGene.

POFUT2 Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC209505 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Pof T2 antibody (TA504972), and then analyzed by flow cytometry.

Product Details

CF504972

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-
Flow Cytometry (Flow)	1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	OTI3A12
Immunogen	Full length human recombinant protein of human POFUT2 produced in HEK293T cell. View antigen
Conjugate	Unconjugated
Form	lyophilized
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.3, with 8% trehalose
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor-like repeats or thrombospondin type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.