|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide between amino acids 100-200 of the human OCT4 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris glycine with 0.15M NaCl|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50-1:200|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody is expected to react with swine, feline and bovine samples based on a 93% sequence homology.
Stem cells are crucial ingredients in the progressive restriction of cellular developmental capacity during mammalian embryogenesis. Until recently, only embryonic stem cells (ESC) had been shown to exhibit the potential to differentiate into cells of the all three germ layers. However, adult stem cells (ASC) can regain pluripotency (potential to differentiate into various lineages) if transferred from one stem-cell microenvironment into another. The transcription factor OCT4, expressed in ESCs and germ cells, is strongly implicated in the process of maintaining as well as regaining stem-cell pluripotency and functions as a key regulator of mammalian germline development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The endometrium of cycling cows contains populations of putative mesenchymal progenitor cells.
PA1-16943 was used in western blot to study the potential existence of mesenchymal progenitor cells in cow endometrium
|Cabezas J,Lara E,Pacha P,Rojas D,Veraguas D,Saravia F,Rodríguez-Alvarez L,Castro FO||Reproduction in domestic animals = Zuchthygiene (49:550)||2014|