Western blot of POU6F1/Brn-5 in human cerebral cortex lysate was performed using 35ug protein in RIPA buffer. Lysates were probed with a POU6F1/Brn-5 polyclonal antibody (Product# PA5-37884) at a dilution of 0.3ug/ml and incubated for 1 hour, followed by chemiluminescent detection.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-ETPTVSQLVSKPHTP, from the internal region of POU6F1|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.3-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with rat, dog and cow based on sequence homology.
Transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3').
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.