Western blot analysis of PPAR was performed by loading 25 ug of HepG2 (lane 1) and NIH-3T3 (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a PPAR polyclonal antibody (Product # PA3-820) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~55 kDa.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: I(484) K K T E T D M S L H P L L Q(498)|
|Storage buffer||whole serum diluted in PBS|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Gel Shift (GS)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:2,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA3-820 detects peroxisome proliferator activated receptor (PPAR) gamma 2 from human, mouse, and rat tissues and cells.
PA3-820 has been successfully used in Western blot, immunoprecipitation and gel shift procedures. By Western blot, this antibody detects an ~55 kDa protein representing PPAR gamma2 from 3T3-L1 cell lysate.
PA3-820 immunizing peptide corresponds to amino acid residues 484-498 from rat PPAR gamma2. This sequence is completely conserved in bovine, chicken, human, mouse, pig and rat PPAR gamma1, PPAR alpha and NUC1.
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPAR and quote;s). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPAR and quote;s are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR and quote;s can induce transcription of acyl coenzyme A oxidase and cytochrome P450 (CYP450) A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with retinoid X receptor (RXR) alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Aspirin inhibits MMP-9 mRNA expression and release via the PPARalpha/gamma and COX-2/mPGES-1-mediated pathways in macrophages derived from THP-1 cells.
PA3-820 was used in western blot to investigate the mechanism of MMP9 expression regulation by aspirin in macrophages
|Xue J,Hua YN,Xie ML,Gu ZL||Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie (64:118)||2010|
Essential role of Smad3 in the inhibition of inflammation-induced PPARbeta/delta expression.
PA3-820 was used in western blot to investigate the role of Smad3 in the inhibition of inflammation-induced PPAR beta/delta expression.
|Tan NS,Michalik L,Di-Poï N,Ng CY,Mermod N,Roberts AB,Desvergne B,Wahli W||The EMBO journal (23:4211)||2004|
A regulatory role for RIP140 in nuclear receptor activation.
PA3-820 was used in western blot to study the role of the RIP140 during the nuclear receptor activation
|Treuter E,Albrektsen T,Johansson L,Leers J,Gustafsson JA||Molecular endocrinology (Baltimore, Md.) (12:864)||1998|
p300 functions as a coactivator for the peroxisome proliferator-activated receptor alpha.
PA3-820 was used in western blot to investigate the interaction of integrator protein (p300) with peroxisome proliferator-activated receptor alpha
|Dowell P,Ishmael JE,Avram D,Peterson VJ,Nevrivy DJ,Leid M||The Journal of biological chemistry (272:33435)||1997|
Differential activation of adipogenesis by multiple PPAR isoforms.
PA3-820 was used in western blot to investigate the differential role of multiple PPAR isoforms on activation of adipogenesis
|Brun RP,Tontonoz P,Forman BM,Ellis R,Chen J,Evans RM,Spiegelman BM||Genes and development (10:974)||1996|
Cyclooxygenase-independent induction of apoptosis by sulindac sulfone is mediated by polyamines in colon cancer.
PA3-820 was used in EMSA to investigate the role of N1-acetyltransferase during the apoptosis induced by sulindac sulfone in human colon cancer cells.
|Babbar N,Ignatenko NA,Casero RA,Gerner EW||The Journal of biological chemistry (278:47762)||2003|
The role of signal transducer and activator of transcription 5 in the inhibitory effects of GH on adipocyte differentiation.
PA3-820 was used in EMSA assay to investigate the role of Janus kinase-2/signal transducer and activator of transcription (STAT)-5 signalling pathway in adipocyte differentiation
|Richter HE,Albrektsen T,Billestrup N||Journal of molecular endocrinology (30:139)||2003|
MCF-7 and T47D human breast cancer cells contain a functional peroxisomal response.
PA3-820 was used in EMSA assay to characterize MCF-7 and T47D human breast cancer cells in terms of peroxisomal response
|Kilgore MW,Tate PL,Rai S,Sengoku E,Price TM||Molecular and cellular endocrinology (129:229)||1997|
Induction and repression of peroxisome proliferator-activated receptor alpha transcription by coregulator ARA70.
PA3-820 was used in immunoprecipitation to investigate the regulatory effect of ARA70 on PPAR alpha transcription
|Heinlein CA,Chang C||Endocrine (21:139)||2003|