Immunofluorescent analysis of PPAR-gamma in 3T3-L1 cells using a PPAR-gamma monoclonal antibody (Product # MA5-14889) (red) showing nuclear localization in differentiated cells. Lipid droplets have been labeled with a BODIPY fluorescent dye (green). DNA is labeled using a fluorescent blue dye.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues surrounding Asp69 of human PPAR-gamma|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:400|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
FoxO1 antagonist suppresses autophagy and lipid droplet growth in adipocytes.
MA5-14889 was used in western blot to learn suppression of autophagy and lipid droplet growth in adipocytes by FoxO1 antagonist
|Liu L,Zheng LD,Zou P,Brooke J,Smith C,Long YC,Almeida FA,Liu D,Cheng Z||Cell cycle (Georgetown, Tex.) (15:2033)||2016|
Tamoxifen reduces fat mass by boosting reactive oxygen species.
MA5-14889 was used in western blot to study the effects of Tamoxifen administration on obesity
|Liu L,Zou P,Zheng L,Linarelli LE,Amarell S,Passaro A,Liu D,Cheng Z||Cell death and disease (6:null)||2015|
Targeting FoxO1 with AS1842856 suppresses adipogenesis.
MA5-14889 was used in western blot to investigate the role of FoxO1 during adipocyte differentiation and adipogenesis
|Zou P,Liu L,Zheng L,Liu L,Stoneman RE,Cho A,Emery A,Gilbert ER,Cheng Z||Cell cycle (Georgetown, Tex.) (13:3759)||2015|
Ebf2 is a selective marker of brown and beige adipogenic precursor cells.
MA5-14889 was used in immunocytochemistry to examine the expression of Ebf2 in adipogenic precursor cells
|Wang W,Kissig M,Rajakumari S,Huang L,Lim HW,Won KJ,Seale P||Proceedings of the National Academy of Sciences of the United States of America (111:14466)||2014|