Immunofluorescent analysis of PPAR-gamma in 3T3-L1 cells using a PPAR-gamma monoclonal antibody (Product # MA5-14889) (red) showing nuclear localization in differentiated cells. Lipid droplets have been labeled with a BODIPY fluorescent dye (green). DNA is labeled using a fluorescent blue dye.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues surrounding Asp69 of human PPAR-gamma|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:400|
|Western Blot (WB)||1:1000|
It is not recommended to aliquot this antibody.
This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.
Tamoxifen reduces fat mass by boosting reactive oxygen species.
MA5-14889 was used in western blot to study the effects of Tamoxifen administration on obesity
|Liu L,Zou P,Zheng L,Linarelli LE,Amarell S,Passaro A,Liu D,Cheng Z||Cell death & disease (6:null)||2015|
Targeting FoxO1 with AS1842856 suppresses adipogenesis.
MA5-14889 was used in western blot to investigate the role of FoxO1 during adipocyte differentiation and adipogenesis
|Zou P,Liu L,Zheng L,Liu L,Stoneman RE,Cho A,Emery A,Gilbert ER,Cheng Z||Cell cycle (Georgetown, Tex.) (13:3759)||2015|
Ebf2 is a selective marker of brown and beige adipogenic precursor cells.
MA5-14889 was used in immunocytochemistry to examine the expression of Ebf2 in adipogenic precursor cells
|Wang W,Kissig M,Rajakumari S,Huang L,Lim HW,Won KJ,Seale P||Proceedings of the National Academy of Sciences of the United States of America (111:14466)||2014|
nuclear receptor subfamily 1 group C member 3; OTTHUMP00000185032; OTTHUMP00000185036; peroxisome proliferator activated receptor gamma; peroxisome proliferator activated receptor gamma 2; peroxisome proliferator activated receptor gamma 4; peroxisome proliferator activator receptor, gamma; peroxisome proliferator-activated nuclear receptor gamma variant 1; peroxisome proliferator-activated receptor gamma; peroxisome proliferator-activated receptor gamma 1; PPAR gamma; PPAR-gamma
CIMT1; GLM1; NR1C3; PPAR-gamma; PPAR-gamma2; PPARG; PPARG1; PPARG2; PPARgamma; PPARgamma2