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|Tested species reactivity||Human, Mouse, Primate|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human PPM1A (amino acids: 202-382) expressed in E. Coli.|
|Storage buffer||PBS with 0.5% proprietary stabilizer|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:200 - 1:400|
|Western Blot (WB)||1:500 - 1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17154 targets PPM1A in FACS, pep-ELISA, and WB applications and shows reactivity with Human and Non-human primate samples.
The MA5-17154 immunogen is purified recombinant fragment of human PPM1A (amino acids: 202-382) expressed in E. Coli.
MA5-17154 detects PPM1A which has a predicted molecular weight of approximately 42.4kDa.
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mpp alpha; PP2C-alpha; protein phosphatase 1A; protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform; protein phosphatase 2C isoform alpha; protein phosphatase IA; protein phosphatase, Mg2+/Mn2+ dependent, 1A
2310003C21Rik; 2900017D14Rik; AI427932; AU017636; MMPa-2; MPPa-1; PP2C-ALPHA; PP2CA; PP2Calpha; PPM1A; PPPM1A