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PPM1E Antibody (MA5-44857) in WB
Western blot analysis of PPM1E in Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Samples were incubated in PPM1E Monoclonal antibody (Product # MA5-44857) using a dilution of 1:500 in 5% BSA at room temperature for 2 hours followed by Goat Anti-Rabbit IgG - HRP secondary antibody at a dilution of 1:200,000 for 1 hour at room temperature.
Product Details
MA5-44857
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE61-81
Immunogen
Synthetic peptide within human PPM1E aa 514-563/755.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2931313
Target Information
Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2. Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. Dephosphorylates PRKAA2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ca(2+)/calmodulin-dependent protein kinase phosphatase N; CaMKN; CaMKP-N; CaMKP-nucleus; nuclear calmodulin-dependent protein kinase phosphatase; Partner of PIX 1; Partner of PIX-alpha; Partner of PIXA; PP2C-eta; Protein phosphatase 1E; protein phosphatase 1E (PP2C domain containing); Protein phosphatase 1M; protein phosphatase 1M (PP2C domain containing); protein phosphatase 2C eta-2; Protein phosphatase 2C isoform eta; protein phosphatase, Mg2+/Mn2+ dependent, 1E; protein phosphatase, Mg2+/Mn2+ dependent, 1M
Gene Aliases: CAMKN; CaMKP-N; KIAA1072; POPX1; PP2C-eta; PP2CE; PP2Ceta; PP2CH; PPM1E; PPM1M
UniProt ID: (Human) Q8WY54, (Human) Q96MI6
Entrez Gene ID: (Human) 22843, (Human) 132160
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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