Western blot analysis of PP1G using anti-PP1G monoclonal antibody (MA5-17250) on (Lane 1) HeLa Cell lysate, (Lane 2) 293T Cell lysate, (Lane 3) Jurket Cell lysate, (Lane 4) K562 Cell lysate, (Lane 5) SK-N-MC Cell lysate, (Lane 6) RD Cell lysate and (Lane 7) IMR32 Cell lysate.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant human protein purified from E.coli (His-PP1G)|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||0.1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is MOLT-4 cells.
Protein phosphatase-1 (PP1) is one of the major Ser/Thr protein phosphatases and is ubiquitous in eukaryotic cells. PP1 is localized to its site of action by interacting with targeting or regulatory proteins, a majority of which contains a primary docking site referred to as the RVXF/W motif. The catalytic subunit of PP1 exists as three isoforms, PP1a, PP1b, and PP1r. The PP1 catalytic subunits are small proteins of 37kDa, which are highly conserved, with their main differences falling in the C-term. The PP1 catalytic subunit exists in the cell in complex with a large number of targeting/regulatory subunits, thereby generating different enzyme forms that allow it to perform a diverse number of cellular functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.