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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1-5 µg/mL | - |
ELISA (ELISA) |
0.1 ng/mL | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Host/Isotype |
Mouse / IgG2b, kappa |
Class |
Monoclonal |
Type |
Antibody |
Clone |
3G11 |
Immunogen |
PPP1R1B (AAH01519, 1 a.a. approximately 168 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
See Label |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.4 |
Contains |
no preservative |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
Sequence of this protein is as follows: MLFRLSEHSS PEEEASPHQR ASGEGHHLKS KRPNPCAYTP PSLKAVQRIA ESHLQSISNL NENQASEEED ELGELRELGY PREEDEEEEE DDEEEEEEED SQAEVLKVIR QSAGQKTTCG QGLEGPWERP PPLDESERDG GSEDQVEDPA LSEPGEEPQR PSPSEPGT
DARPP-32 is a dopamine (DA) and AMP-regugated ~32 kDa phosphoprotein that is associated with dopaminoceptive neurons. The protein inhibits Protein Phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP-32 is thought to play a critical role in the regugation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac®.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DARPP-32; dopamine and cAMP-regulated neuronal phosphoprotein 32; Dopamine- and cAMP-regulated neuronal phosphoprotein; FLJ20940; IPPD; OTTHUMP00000164275; OTTHUMP00000164276; PPR1B; protein pho; Protein phosphatase 1 regulatory subunit 1B
Gene Aliases: DARPP-32; DARPP32; PPP1R1B
UniProt ID: (Human) Q9UD71
Entrez Gene ID: (Human) 84152
Molecular Function:
phosphatase inhibitor
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