Western blot analysis of PP2A in NIH-3T3 Puro lysates using a PPP2CA/PP2A alpha monoclonal antibody (Product # MA5-18060) at various dilutions, followed by detection using an AP-conjugated mouse IgG whole molecule antibody at a dilution of 1:2000 on a BCIP/NBT substrate.
|Tested species reactivity||Human, Mouse, Rat, Yeast|
|Host / Isotype||Mouse / IgG1|
|Immunogen||C-terminal peptide of human PP2A catalytic subunit|
|Contains||0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2000-1:4000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody recognizes the C-terminal of PP2A, both alpha and beta isoforms, in human, mouse, rat and S.cerevisiae samples.
The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a gamma isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.