Western blot analysis of PPP2CA / PPP2CB in Mouse (A), Rat (B) and Pig (C) Brain lysates (35µg protein in RIPA buffer). Samples were probed with the PPP2CA / PPP2CB antibody (Product # PA5-17980, 0.1µg / ml ) for 1 hour. Western blot was detected by chemiluminescence.
|Tested species reactivity||Human, Mouse, Pig, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide sequence (EPHVTRRTPDYFL) corresponding to the C-terminus amino acids of PPP2CA / PPP2CB|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.05-0.2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, canine, mouse, porcine, rabbit and rat based on sequence homology.
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.
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