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Invitrogen
PPP2R5D Monoclonal Antibody (H5D12)
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FIGURE: 1 / 7
PPP2R5D Antibody (MA5-18066) in ICC/IF
Immunocytochemistry analysis of PPP2R5D in paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Samples were incubated with PPP2R5D monoclonal antibody (Product # MA5-18066) using a dilution of 1:100 for 1hr. Followed by Alexa Fluor® 488 secondary antibody at a 1:1,000 dilution, showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Mouse IgG1 negative control followed by Alexa Fluor® 488 secondary antibody.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-18066
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
H5D12
Immunogen
GST-N-terminal fragment of human PP2A-B56 delta (Immunogen sequence: 2-165 (GST fusion))
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody does not exhibit cross-reactivity with B56 alpha, beta, gamma or epsilon.
Target Information
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZNF265 (Zinc finger protein 265), also known as ZRANB2 (Zinc finger Ran-binding domain-containing protein 2), ZIS, ZIS1 or ZIS2, is a 330 amino acid protein that belongs to the ZRANB2 family. Localized to the nucleus, ZNF265 functions as a splicing factor that is responsible for alternatively splicing Tra-2 beta (transformer-2 beta) transcripts and is thought to interfere with constitutive 5'-splice selection. ZNF265 contains two RanBP2-type zinc fingers through which it conveys its RNA-binding activity. Two isoforms, designated ZIS-1 and ZIS-2, are expressed due to alternative splicing events. Upon DNA damage, ZIS-2 may be phosphorylated by ATM or ATR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: MGC2134; MGC8949; PP2CB; unnamed protein product
Gene Aliases: B56D; B56delta; HJS1; MRD35
UniProt ID: (Human) Q14738
Entrez Gene ID: (Human) 5528, (Rat) 363193
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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