Invitrogen

PPT1 Monoclonal Antibody (OTI1F10)

Product Image Gallery

PPT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human breast tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a PPT1 monoclonal antibody (Product # MA5-26471).

PPT1 Antibody in Western Blot (WB) — Western blot analysis of PPT1 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with PPT1 (Product # MA5-26471) monoclonal antibody.

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1:500

Western Blot (WB)

1:2000

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI1F10

Immunogen

Human recombinant protein fragment corresponding to amino acids 100-306 of PPT1 produced in E.coli

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2725101

Target Information

Palmitoyl-protein thioesterase-1 (PPT1) is a lysosomal hydrolase that removes long-chain fatty acyl groups from modified cysteine residues in proteins. Mutations in PPT1 have been found to cause the infantile form of neuronal ceroid lipofuscinosis (INCL), and an animal model has been developed.1 The deduced PPT2 protein contains 302 amino acids, including a 27-amino acid leader peptide, a sequence motif characteristic of many thioesterases and lipases, and 5 potential N-linked glycosylation sites.2 PPT2 shares 18% amino acid identity with PPT1. Northern blot analysis detected a predominant 2.0-kb PPT2 transcript in the human tissues examined, with the highest expression in skeletal muscle; variable amounts of 2.8- and 7.0-kb transcripts were also observed. Recombinant PPT2, like PPT1, possesses thioesterase activity and localizes to the lysosome. Since PPT2 could not substitute for PPT1 in correcting the metabolic defect in INCL cells and was unable to remove palmitate groups from palmitoylated proteins that are routinely used as substrates for PPT1it has been postulated that PPT2 possesses a different substrate specificity than PPT1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ceroid-palmitoyl-palmitoyl-protein thioesterase 1; CLN1; INCL; Palmitoyl-protein hydrolase 1; Palmitoyl-protein thioesterase 1; PPT-1

Gene Aliases: CLN1; INCL; PPT; PPT1

UniProt ID: (Human) P50897

Entrez Gene ID: (Human) 5538

Molecular Function: esterase hydrolase