Immunohistochemical analysis of paraffin-embedded human liver carcinoma (A), esophagus carcinoma (B), normal spleen tissue (C), breast carcinoma (D), showing nuclear and cytoplasmic localization using PRAK monoclonal antibody (Product # MA5-15352) followed with DAB staining.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of PRAK expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-15352 targets PRAK in IHC and WB applications and shows reactivity with Human samples.
The MA5-15352 immunogen is purified recombinant fragment of PRAK expressed in E. Coli.
The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.
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