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Immunohistochemical analysis of paraffin-embedded human liver carcinoma (A), esophagus carcinoma (B), normal spleen tissue (C), breast carcinoma (D), showing nuclear and cytoplasmic localization using PRAK monoclonal antibody (Product # MA5-15352) followed with DAB staining.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of PRAK expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-15352 targets PRAK in IHC and WB applications and shows reactivity with Human samples.
The MA5-15352 immunogen is purified recombinant fragment of PRAK expressed in E. Coli.
The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAPK-5; MAPKAPK5; p38-regulated/activated protein kinase; PRAK
MAPKAP-K5; MAPKAPK5; MK-5; MK5; PRAK