|Western Blot (WB)||1:100-1:1000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to amino acids 2–256 of human PRAS40.|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Proline Rich Akt Substrate of 40 kDa (PRAS40) is a signaling target known to play a regulatory role in growth, development, and apoptosis through the phosphoinositol kinase 3 pathway. PRAS40 also acts by binding to mTOR complex 1 and inhibiting mTOR function by altering nutrient balance, and growth maintenance. Phosphorylation of PRAS40 by mTORC1 at Serine 183, 212 and 221 residues disrupts this binding thus removing the inhibitory effect. Activated Akt phosphorylates PRAS40 on threonine 246. Mutation of PRAS40 threonine 246 has been shown to be apoptotic, and plays a role in abrogating neuronal protection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 40 kDa; 40 kDa proline-rich AKT substrate; AKT1 substrate 1 (proline rich); AKT1 substrate 1 (proline-rich); AKT1S1; OTTHUMP00000196756; OTTHUMP00000196760; proline-rich Akt substrate; Proline-rich AKT1 substrate 1
Gene Aliases: AKT1S1; Lobe; PRAS40
UniProt ID: (Human) Q96B36
Entrez Gene ID: (Human) 84335
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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