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Immunofluorescence analysis of PRAS40 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with PRAS40 Mouse monoclonal Antibody (AHO1031) at 2µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour 488 Rabbit Anti-Mouse IgG Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing cytoplasmic localization. Panel e shows no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant full length human PRAS40 expressed in E. coli.|
|Storage buffer||PBS with 1% BSA|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals. AKT1S1, also known as PRAS40, is a proline-rich substrate of the kinase AKT1 and is thought to play a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia. AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Treatment with the insulin-like growth factor-1 (IGF1) can indusce the phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Combination of heat shock protein 90 and focal adhesion kinase inhibitors synergistically inhibits the growth of non-small cell lung cancer cells.
AHO1031 was used in western blot to assess the impact of heat shock protein 90 inhibition in combination with focal adhesion kinase inhibitor on the growth of non-small cell lung cancer cells.
|Webber PJ,Park C,Qui M,Ramalingam SS,Khuri FR,Fu H,Du Y||Oncoscience (2:765)||2015|
Characterization of the activity of the PI3K/mTOR inhibitor XL765 (SAR245409) in tumor models with diverse genetic alterations affecting the PI3K pathway.
AHO1031 was used in western blot to study the anti-tumor effects of a pan class I PI3 kinase/mTOR inhibitor in cells with a genetically diverse range of PI3 kinase pathway modifications.
|Yu P,Laird AD,Du X,Wu J,Won KA,Yamaguchi K,Hsu PP,Qian F,Jaeger CT,Zhang W,Buhr CA,Shen P,Abulafia W,Chen J,Young J,Plonowski A,Yakes FM,Chu F,Lee M,Bentzien F,Lam ST,Dale S,Matthews DJ,Lamb P,Foster P||Molecular cancer therapeutics (13:1078)||2014|
Effects of adding exercise to a 16-week very low-calorie diet in obese, insulin-dependent type 2 diabetes mellitus patients.
||Snel M,Gastaldelli A,Ouwens DM,Hesselink MK,Schaart G,Buzzigoli E,Frölich M,Romijn JA,Pijl H,Meinders AE,Jazet IM||The Journal of clinical endocrinology and metabolism (97:2512)||2012|
40 kDa proline-rich AKT substrate, proline-rich AKT1 substrate 1, OTTHUMP00000196756, OTTHUMP00000196760, proline-rich Akt substrate, 40 kDa