|Tested species reactivity||Human, Mouse, Pig|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||A fragment of the mouse Blimp-1 (residues 199-409).|
|Storage buffer||tris glycine with 0.15M NaCl|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1µg antibody / 10 µg protein|
|Flow Cytometry (Flow)||8 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Western Blot (WB)||1:1000|
|Immunohistochemistry (IHC)||See 1 publications below|
In Western Blot, this antibody recognizes a band at ~98 kDa and may recognize one at ~80 kDa (the beta form).
Antigen retrieval is recommended (EDTA buffer, microwave) prior to IHC on paraffin tissues.
This antibody does not immunoprecipitate.For IHC and ICC/IF a dilution of 1:200 is recommended with tyramide amplification; this antibody demonstrates nuclear staining.
Suggested positive control: antigen standard for PRDM1 (transient overexpression lysate), P3X (murine plasmocytoma) cell lysates.
Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.
Critical function of AP-2 gamma/TCFAP2C in mouse embryonic germ cell maintenance.
MA1-16874 was used in immunohistochemistry to investigate the roles of AP-2 gamma/TCFAP2C in mouse embryonic germ cells
|Weber S,Eckert D,Nettersheim D,Gillis AJ,Schäfer S,Kuckenberg P,Ehlermann J,Werling U,Biermann K,Looijenga LH,Schorle H||Biology of reproduction (82:214)||2010|
B Lymphocyte Induced Maturation Protein 1; B-lymphocyte-induced maturation protein 1; beta-interferon gene positive-regulatory domain I binding factor; BLIMP 1; BLIMP-1; BLIMP1; Positive Regulatory Domain I Binding Factor 1; PR Domain Containing 1; PR domain containing 1, with ZNF domain; PRDI BF1; PRDI-binding factor-1; PRDM1
b2b1765Clo; Blimp-1; BLIMP1; PRDI-BF1; PRDM1; ZNFPR1A1