OriGene

PRKD2 Monoclonal Antibody (OTI4F1), TrueMAB™

View all (35) PRKD2 antibodies

Questions & Answers

Questions & Answers

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PRKD2 Antibody in Immunocytochemistry (ICC/IF) — Anti-PRKD2 mouse monoclonal antibody (TA501684) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKD2(RC215335).

PRKD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human bladder tissue within the normal limits using anti-PRKD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501684, Dilution 1:50)

PRKD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PRKD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501684, Dilution 1:50)

PRKD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PRKD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501684, Dilution 1:50)

PRKD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PRKD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501684, Dilution 1:50)

PRKD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human lymphoma tissue using anti-PRKD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501684, Dilution 1:50)

PRKD2 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKD2 (RC215335, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKD2. Positive lysates LY402555 (100 µg) and LC402555 (20 µg) can be purchased separately from OriGene.

PRKD2 Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC215335 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRKD2 antibody (TA501684), and then analyzed by flow cytometry.

Product Details

TA501684

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-
Immunocytochemistry (ICC/IF)	1:100	-
Flow Cytometry (Flow)	1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	OTI4F1
Immunogen	Full length human recombinant protein of human PRKD2 produced in HEK293T cell. View antigen
Conjugate	Unconjugated
Form	liquid
Concentration	0.89 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Target Information

Protein kinase D2 (PKD2, PRKD2) is a novel human serine threonine protein kinase gene. It exhibits the strongest homology to the serine threonine protein kinases PKD/PKC and PKCnu, particularly in the duplex zinc finger-like cysteine-rich motif, in the pleckstrin homology domain and in the protein kinase domain. In contrast, it shows only a low degree of sequence similarity to other members of the PKC family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: nPKC-D2; Serine/threonine-protein kinase D2

Gene Aliases: HSPC187; nPKC-D2; PKD2; PRKD2

UniProt ID: (Human) Q9BZL6

Entrez Gene ID: (Human) 25865

Molecular Function: non-receptor serine/threonine protein kinase

angiogenesis positive regulation of endothelial cell proliferation adaptive immune response protein phosphorylation cell adhesion cell death positive regulation of endothelial cell migration peptidyl-serine phosphorylation positive regulation of vascular endothelial growth factor receptor signaling pathway positive regulation of interleukin-2 production positive regulation of interleukin-8 production positive regulation of CREB transcription factor activity positive regulation of peptidyl-serine phosphorylation intracellular signal transduction cellular response to vascular endothelial growth factor stimulus positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway positive regulation of blood vessel endothelial cell migration positive regulation of fibroblast growth factor receptor signaling pathway positive regulation of angiogenesis positive regulation of cell adhesion positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation vascular endothelial growth factor receptor signaling pathway T cell receptor signaling pathway positive regulation of T cell receptor signaling pathway positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity endothelial tube morphogenesis positive regulation of ERK1 and ERK2 cascade protein kinase D signaling mitophagy in response to mitochondrial depolarization positive regulation of histone deacetylase activity positive regulation of intracellular signal transduction positive regulation of DNA biosynthetic process positive regulation of endothelial cell chemotaxis

Process(es)

protein kinase activity protein serine/threonine kinase activity protein kinase C activity protein binding ATP binding metal ion binding