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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human PRKDC expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1/200 - 1/1000|
|Immunofluorescence (IF)||1:200 - 1:1000|
|Immunohistochemistry (IHC)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
MA5-15813 targets PRKDC in ICC, IHC, IF and WB applications and shows reactivity with Human samples.
The MA5-15813 immunogen is purified recombinant fragment of human PRKDC expressed in E. Coli.
MA5-15813 detects PRKDC which has a predicted molecular weight of approximately 46.9kDa.
The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku, which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A localized nucleolar DNA damage response facilitates recruitment of the homology-directed repair machinery independent of cell cycle stage.
MA5-15813 was used in immunocytochemistry to study the response to double-stranded breaks at the nucleolar organizer regions.
|van Sluis M,McStay B||Genes and development (29:1151)||2015|
DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs, DNAPK, DNPK1, HYRC, HYRC1, XRCC7, p350, DNA-PK catalytic subunit; DNPK1; hyper-radiosensitivity of murine scid mutation, complementing 1; p460
DNA-PKcs; DNAPK; DNPK1; HYRC; HYRC1; IMD26; p350; PRKDC; XRCC7