Immunofluorescent analysis of PRKR in HEK293 cells using a PRKR recombinant rabbit monoclonal antibody (Product # 700286) at a dilution of 5ug/ml followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000. Cells were fixed using 4% paraformaldehyde. Cytoplasmic localization of PKR specific signal is shown in green, while nuclei were stained using SlowFade GOLD with DAPI (Product # S36938) shown in blue.
|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A recombinant protein corresponding to amino acids 54-158.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5 ug/ml|
|Immunofluorescence (IF)||5 ug/ml|
|Western Blot (WB)||1-3 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
This antibody is predicted to react with Rhesus monkey based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Double-stranded RNA-dependent protein kinase (PKR) is a 68 kDa protein that is induced by interferon and double-stranded RNAs (dsRNA) produced in virus-infected cells. Two dsRNA-binding domains in the N-terminus interact with dsRNA to modify the conformation of PKR and allow it to undergo autophosphorylation and activation. Once activated, PKR phosphorylates eIF2 alpha, leading to inhibition of protein synthesis, growth suppression, and apoptosis induction. The phosphorylation of two sites in the activation loop of the kinase domain, threonines 446 and 451 is critical for high level catalytic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Opposing Roles of Double-Stranded RNA Effector Pathways and Viral Defense Proteins Revealed with CRISPR-Cas9 Knockout Cell Lines and Vaccinia Virus Mutants.
700286 was used in western blot to utilize CRISPR/Cas9 knock-out cell lines and vaccinia virus mutants to elucidate opposing roles of double-stranded RNA effector pathways and viral defense proteins
|Liu R,Moss B||Journal of virology (90:7864)||2016|
|Not Applicable||Not Cited||
Post-translational Regulation of Hexokinase Function and Protein Stability in the Aestivating Frog Xenopus laevis.
700286 was used in western blot to utilize an Aestivating Frog Xenopus laevis model to study post-translation regulation of hexokinase function and protein stability
|Childers CL,Storey KB||The protein journal (35:61)||2016|