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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues 500-550 of mouse PRKR.|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: Daudi cell lysate.
PRKR, a 68KDa protein, is an IFN-induced serine/threonine protein kinase consisting of two N-terminal RNA-binding regulatory domains and a functional C-terminal serine/threonine protein kinase domain. Activated by dsRNA produced during viral infections, it plays a key role in IFN induced-innate antiviral response, virus-induced apoptosis, cell growth & differentiation. Upon activation, PRKR gets autophosphorylated and catalyzes the phosphorylation of elF2 alpha subunit, which leads to an inhibition of protein synthesis. It induces apoptosis by up-regulating Fas expression and mediates FADD/Caspase-8 death signaling pathway. Upon viral infection, it functions as a dual protein, sequentially activating both cell survival & cell death pathways using kinase independent and dependent strategies. PRKR regulates multiple pathways which include NF-kappaB activation, p53, p38, & PDGF signaling pathway. Its role has been implicated in tumor suppression & malignancy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
double stranded RNA activated protein kinase; eIF-2A protein kinase 2; EIF2AK1; eukaryotic translation initiation factor 2-alpha kinase 2; interferon-inducible elF2alpha kinase; MGC126524; P1/eIF-2A protein kinase; p68 kinase; PKR; PRKR; protein kinase R; protein kinase, interferon-inducible double stranded RNA dependent; protein phosphatase 1, regulatory subunit 83; tyrosine-protein kinase EIF2AK2
EIF2AK1; EIF2AK2; PKR; PPP1R83; PRKR