Invitrogen

PRKRIP1 Monoclonal Antibody (4D11-3F11)

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PRKRIP1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of HeLa cells using an anti-PRKRIP1 monoclonal antibody (Product # MA5-22590). (antibody concentration 10 µg/mL).

PRKRIP1 Antibody in Western Blot (WB) — Western blot analysis of PRKRIP1 in cell lysate from Hela S3 NE cells, using a anti-PRKRIP1 monoclonal antibody (Product # MA5-22590).

PRKRIP1 Antibody in ELISA (ELISA) — Detection limit for recombinant GST tagged PRKRIP1 using anti-PRKRIP1 monoclonal antibody (Product # MA5-22590) is approximately 3 ng/mL as a capture antibody.

Product Details

MA5-22590

Applications	Tested Dilution	Publications
Western Blot (WB)	1-5 µg/mL	-
Immunocytochemistry (ICC/IF)	10 µg/mL	-
ELISA (ELISA)	Assay-Dependent	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	4D11-3F11
Immunogen	PRKRIP1 (AAH14298, 1 a.a. ~ 184 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2577262

Product Specific Information

Peptide Sequence: MASPAASSVR PPRPKKEPQT LVIPKNAAEE QKLKLERLMK NPDKAVPIPE KMSEWAPRPP PEFVRDVMGS SAGAGSGEFH VYRHLRRREY QRQDYMDAMA EKQKLYAEFQ KRLEKNKIAA EEQTAKRRKK RQKLKEKKLL AKKMKLEQKK QEGPGQPKEQ GSSSSAEASG TEEEEEVPSF TMGR

Target Information

Required for pre-mRNA splicing as component of the spliceosome (PubMed:28502770, PubMed:30705154). Binds double-stranded RNA. Inhibits EIF2AK2 kinase activity. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.