Invitrogen

PRMT5 Monoclonal Antibody (PRMT5-21)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 Published Figures
2 References
View all (39) PRMT5 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA1-25470

Applications
Tested Dilution
Publications

Western Blot (WB)

2 µg/mL
View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent
-

ChIP assay (ChIP)

2.5 µg/10^6 cells
View 1 publication 1 publication
Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Hamster, Human, Mouse, Non-human primate, Rat

Published species

Human, Zebrafish

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

PRMT5-21

Immunogen

Fusion of mouse myeloma cells and splenocytes from mice immunized with PRMT5 containing the C-terminal region of the Human PRMT5 (residues 315-637).
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.4 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2253071

Product Specific Information

Recommended positive controls: G361.

Target Information

PRMT5 (protein arginine N-methyltransferase 5) is an arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3; such methylation is required for the assembly and biogenesis of snRNP core particles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 72 kDa ICln-binding protein; EC 2.1.1.125; Histone-arginine N-methyltransferase PRMT5; HMT1 hnRNP methyltransferase-like 5; Jak-binding protein 1; PRMT5; protein arginine methyltransferase 5; Protein arginine N-methyltransferase 5; protein arginine N-methyltransferase 5-like; Shk1 kinase-binding protein 1 homolog; SKB1 homolog

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Gene Aliases: HRMT1L5; I79_013398; IBP72; JBP1; PRMT5; SKB1; SKB1Hs

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UniProt ID: (Human) O14744, (Bovine) A7YW45, (Mouse) Q8CIG8

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Entrez Gene ID: (Human) 10419, (Bovine) 515594, (Chicken) 101750306, (Dog) 480242, (Mouse) 27374, (Rat) 364382, (Chinese hamster) 100768836

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Function(s)
core promoter sequence-specific DNA binding transcription corepressor activity protein binding methyltransferase activity methyl-CpG binding histone-arginine N-methyltransferase activity protein-arginine N-methyltransferase activity protein complex binding protein-arginine omega-N symmetric methyltransferase activity ribonucleoprotein complex binding histone methyltransferase activity (H4-R3 specific) protein heterodimerization activity E-box binding rRNA (adenine-N6,N6-)-dimethyltransferase activity chromatin binding protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity protein modifying enzyme
Process(es)
spliceosomal snRNP assembly DNA-templated transcription, termination regulation of transcription, DNA-templated regulation of mitotic nuclear division cell proliferation peptidyl-arginine methylation peptidyl-arginine methylation, to symmetrical-dimethyl arginine circadian regulation of gene expression peptidyl-arginine N-methylation endothelial cell activation histone H4-R3 methylation regulation of DNA methylation negative regulation of cell differentiation positive regulation of oligodendrocyte differentiation regulation of ERK1 and ERK2 cascade Golgi ribbon formation liver regeneration regulation of signal transduction by p53 class mediator negative regulation of nucleic acid-templated transcription positive regulation of adenylate cyclase-inhibiting dopamine receptor signaling pathway negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated protein methylation chromatin modification methylation histone arginine methylation rhythmic process
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