Invitrogen
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
2 µg/mL | View 1 publication 1 publication |
ELISA (ELISA) |
Assay-dependent | - |
ChIP assay (ChIP) |
2.5 µg/10^6 cells | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Bovine, Dog, Chicken, Hamster, Human, Mouse, Non-human primate, Rat |
Published species |
Zebrafish |
Host/Isotype |
Mouse / IgG2a |
Class |
Monoclonal |
Type |
Antibody |
Clone |
PRMT5-21 |
Immunogen |
Fusion of mouse myeloma cells and splenocytes from mice immunized with PRMT5 containing the C-terminal region of the Human PRMT5 (residues 315-637). |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
2.4 mg/mL |
Purification |
Protein A |
Storage buffer |
0.01M PBS, pH 7.4 |
Contains |
15mM sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2253071 |
Recommended positive controls: G361.
PRMT5 (protein arginine N-methyltransferase 5) is an arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3; such methylation is required for the assembly and biogenesis of snRNP core particles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 72 kDa ICln-binding protein; EC 2.1.1.125; Histone-arginine N-methyltransferase PRMT5; HMT1 hnRNP methyltransferase-like 5; Jak-binding protein 1; PRMT5; protein arginine methyltransferase 5; Protein arginine N-methyltransferase 5; Protein arginine N-methyltransferase 5, N-terminally processed; protein arginine N-methyltransferase 5-like; Shk1 kinase-binding protein 1 homolog; SKB1 homolog
Gene Aliases: HRMT1L5; I79_013398; IBP72; JBP1; PRMT5; SKB1; SKB1Hs
UniProt ID: (Human) O14744, (Bovine) A7YW45, (Mouse) Q8CIG8
Entrez Gene ID: (Human) 10419, (Bovine) 515594, (Chicken) 101750306, (Dog) 480242, (Mouse) 27374, (Rat) 364382, (Chinese hamster) 100768836
Molecular Function:
protein modifying enzyme
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