Invitrogen

PRMT5 Monoclonal Antibody (PRMT5-21)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 Published Figures

2 References

View all (32) PRMT5 antibodies

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Datasheet

Protocols

Questions & Answers

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PRMT5 Antibody in Western Blot (WB) — Knockdown of PRMT5 was achieved by transfecting HeLa cells with PRMT5 specific siRNAs (Silencer® select Product # s20377). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) from the PRMT5 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with PRMT5 Monoclonal Antibody (PRMT1) (Product # MA1-25470, 2 µg/mL) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PRMT5.

PRMT5 Antibody in ChIP assay (ChIP) — Enrichment of endogenous PRMT5 protein at specific gene loci using Anti-PRMT5 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-PRMT5 Mouse Monoclonal antibody (Product # MA1-25470, 5 µg) on sheared chromatin from 2 million HCT 116 cells using the MAGnify ChIP System (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over the promoters of ADIPOQ, BIRC3, CCNE1, FOXP1 (positive) and SAT2 satellite repeats (negative). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

PRMT5 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with PRMT5 siRNA and reduction of signal was observed in Western Blot using PRMT5 Monoclonal Antibody (PRMT521) (Product # MA1-25470). {KD}

Product Details

MA1-25470

Applications	Tested Dilution	Publications
Western Blot (WB)	2 µg/mL	View 1 publication 1 publication
ELISA (ELISA)	Assay-dependent	-
ChIP assay (ChIP)	2.5 µg/10^6 cells	View 1 publication 1 publication

Product Specifications
Species Reactivity	Bovine, Dog, Chicken, Hamster, Human, Mouse, Non-human primate, Rat
Published species	Zebrafish
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	PRMT5-21
Immunogen	Fusion of mouse myeloma cells and splenocytes from mice immunized with PRMT5 containing the C-terminal region of the Human PRMT5 (residues 315-637). View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	2.4 mg/mL
Purification	Protein A
Storage buffer	0.01M PBS, pH 7.4
Contains	15mM sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2253071

Product Specific Information

Recommended positive controls: G361.

Target Information

PRMT5 (protein arginine N-methyltransferase 5) is an arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3; such methylation is required for the assembly and biogenesis of snRNP core particles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Prmt5 promotes vascular morphogenesis independently of its methyltransferase activity.

PLoS genetics

Quillien A,Gilbert G,Boulet M,Ethuin S,Waltzer L,Vandel L

MA1-25470 was used in ChIP assay to study the role of the Protein Arginine Methyl Transferase Prmt5 during blood vessel formation and hematopoiesis in a zebrafish model.

Tue Jun 01 00:00:00 EDT 2021

Protein arginine methyltransferase 5 (PRMT5) activates WNT/β-catenin signalling in breast cancer cells via epigenetic silencing of DKK1 and DKK3.

Journal of cellular and molecular medicine

Shailesh H,Siveen KS,Sif S

Published figure using PRMT5 monoclonal antibody (Product # MA1-25470) in Western Blot

Mon Feb 01 00:00:00 EST 2021

Bioinformatics

Protein Aliases: 72 kDa ICln-binding protein; EC 2.1.1.125; Histone-arginine N-methyltransferase PRMT5; HMT1 hnRNP methyltransferase-like 5; Jak-binding protein 1; PRMT5; protein arginine methyltransferase 5; Protein arginine N-methyltransferase 5; Protein arginine N-methyltransferase 5, N-terminally processed; protein arginine N-methyltransferase 5-like; Shk1 kinase-binding protein 1 homolog; SKB1 homolog

Gene Aliases: HRMT1L5; I79_013398; IBP72; JBP1; PRMT5; SKB1; SKB1Hs

UniProt ID: (Human) O14744, (Bovine) A7YW45, (Mouse) Q8CIG8

Entrez Gene ID: (Human) 10419, (Bovine) 515594, (Chicken) 101750306, (Dog) 480242, (Mouse) 27374, (Rat) 364382, (Chinese hamster) 100768836

Molecular Function: protein modifying enzyme