Invitrogen

PRMT5 Monoclonal Antibody (PRMT5-21)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 Published Figures

2 References

View all (34) PRMT5 antibodies

Datasheet

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Datasheet

Protocols

Questions & Answers

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FIGURE: 1 / 7

PRMT5 Antibody (MA1-25470) in WB

Western blot analysis was performed on modified whole cell extracts (30 µg lysate) of HeLa (Lane 1), T-47D (Lane 2), Caco-2 (Lane 3), HepG2 (Lane 4), PC-3 (Lane 5) and NIH/3T3 (Lane 6). The blot was probed with Anti-PRMT5 Monoclonal Antibody (PRMT5-21) (Product # MA1-25470, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 72 kDa band corresponding to PRMT5 was observed in all human and mouse cell line(s) test... View More

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PRMT5 Antibody in ChIP Assay (ChIP) — Enrichment of endogenous PRMT5 protein at specific gene loci using Anti-PRMT5 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-PRMT5 Mouse Monoclonal antibody (Product # MA1-25470, 5 µg) on sheared chromatin from 2 million HCT 116 cells using the MAGnify ChIP System (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over the promoters of ADIPOQ, BIRC3, CCNE1, FOXP1 (positive) and SAT2 satellite repeats (negative). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

PRMT5 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with PRMT5 siRNA and reduction of signal was observed in Western Blot using PRMT5 Monoclonal Antibody (PRMT521) (Product # MA1-25470). {KD}

Product Details

MA1-25470

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

ChIP assay (ChIP)

2.5 µg/10^6 cells

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Hamster, Human, Mouse, Non-human primate, Rat

Published species

Zebrafish

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

PRMT5-21

Immunogen

Fusion of mouse myeloma cells and splenocytes from mice immunized with PRMT5 containing the C-terminal region of the Human PRMT5 (residues 315-637).

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

2.4 mg/mL

Purification

purified

Storage buffer

0.01M PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2253071

Product Specific Information

Recommended positive controls: G361.

Target Information

PRMT5 (protein arginine N-methyltransferase 5) is an arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3; such methylation is required for the assembly and biogenesis of snRNP core particles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Protein arginine methyltransferase 5 (PRMT5) activates WNT/β-catenin signalling in breast cancer cells via epigenetic silencing of DKK1 and DKK3.

Journal of cellular and molecular medicine

Shailesh H,Siveen KS,Sif S

Published figure using PRMT5 monoclonal antibody (Product # MA1-25470) in Western Blot

Mon Feb 01 00:00:00 EST 2021

Prmt5 promotes vascular morphogenesis independently of its methyltransferase activity.

PLoS genetics

Quillien A,Gilbert G,Boulet M,Ethuin S,Waltzer L,Vandel L

MA1-25470 was used in ChIP assay to study the role of the Protein Arginine Methyl Transferase Prmt5 during blood vessel formation and hematopoiesis in a zebrafish model.

Tue Jun 01 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: 72 kDa ICln-binding protein; EC 2.1.1.125; Histone-arginine N-methyltransferase PRMT5; HMT1 hnRNP methyltransferase-like 5; Jak-binding protein 1; PRMT5; protein arginine methyltransferase 5; Protein arginine N-methyltransferase 5; Protein arginine N-methyltransferase 5, N-terminally processed; protein arginine N-methyltransferase 5-like; Shk1 kinase-binding protein 1 homolog; SKB1 homolog

Gene Aliases: HRMT1L5; I79_013398; IBP72; JBP1; PRMT5; SKB1; SKB1Hs

UniProt ID: (Human) O14744, (Bovine) A7YW45, (Mouse) Q8CIG8

Entrez Gene ID: (Human) 10419, (Bovine) 515594, (Chicken) 101750306, (Dog) 480242, (Mouse) 27374, (Rat) 364382, (Chinese hamster) 100768836

Molecular Function: protein modifying enzyme