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Invitrogen
PRMT6 Recombinant Rabbit Monoclonal Antibody (8H9L19)
FIGURE: 1 / 4
PRMT6 Antibody (703547) in ICC/IF
For immunofluorescence analysis, MCF7 cells were fixed and permeabilized for detection of endogenous PRMT6 using Anti-PRMT6 Recombinant Rabbit Monoclonal Antibody (Product # 703547, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Panel a-d)... View More
Product Details
703547
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
8H9L19
Immunogen
Peptides corresponding to human PRMT6 [1) aa37-aa49 2) aa358-aa375]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809240
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Rat, Cat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Protein arginine N-methyltransferase 6 (PRMT6) PRMT6 demonstrates type I PRMT activity, capable of forming both omega-N monomethylarginine and asymmetric dimethylarginine derivatives on the recombinant glycine- and arginine-rich substrates. cts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1. Promotes fasting-induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ10559; FLJ51477; Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6; Histone-arginine N-methyltransferase PRMT6; HMT1 hnRNP methyltransferase-like 6; PRMT-6; Protein arginine N-methyltransferase 6
Gene Aliases: HRMT1L6; PRMT6
UniProt ID: (Human) Q96LA8
Entrez Gene ID: (Human) 55170
chromatin binding
protein binding
histone-arginine N-methyltransferase activity
protein-arginine N-methyltransferase activity
protein-arginine omega-N monomethyltransferase activity
protein-arginine omega-N asymmetric methyltransferase activity
histone methyltransferase activity
histone binding
histone methyltransferase activity (H4-R3 specific)
histone methyltransferase activity (H3-R2 specific)
histone methyltransferase activity (H2A-R3 specific)
protein modifying enzyme
negative regulation of transcription from RNA polymerase II promoter
base-excision repair
transcription, DNA-templated
viral process
cell growth
histone methylation
peptidyl-arginine methylation, to asymmetrical-dimethyl arginine
histone H3-R2 methylation
histone H4-R3 methylation
negative regulation of transcription, DNA-templated
cellular senescence
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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