Invitrogen

PRP19 Recombinant Rabbit Monoclonal Antibody (SC06-53)

View all (19) PRP19 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

PRP19 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of PRP19 in B16-F1 cells using a PRP19 Monoclonal antibody (Product # MA5-32222) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

PRP19 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of PRP19 of paraffin-embedded Human breast carcinoma tissue using a PRP19 Monoclonal antibody (Product #MA5-32222). Counter stained with hematoxylin.

PRP19 Antibody in Western Blot (WB) — Western blot analysis of PRP19 in Jurkat cell lysates using a PRP19 Monoclonal antibody (Product # MA5-32222) at a dilution of 1:1,000.

PRP19 Antibody in Flow Cytometry (Flow) — Flow Cytometric analysis of PRP19 in Hela cells using a PRP19 Monoclonal Antibody (Product # MA5-32222) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Product Details

MA5-32222

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

Immunohistochemistry (IHC)

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50-1:200

Flow Cytometry (Flow)

1:50-1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SC06-53

Immunogen

recombinant protein within Human PRP19 aa 380-524

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2809508

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

PSO4 is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: hPso4; Neuronal differentiation-related gene protein; Nuclear matrix protein 200; nuclear matrix protein NMP200 related to splicing factor PRP19; nuclear matrix protein SNEV; Pre-mRNA-processing factor 19; prp19 beta; PRP19/PSO4 homolog; PRP19/PSO4 pre-mRNA processing factor 19 homolog; psoralen 4; RING-type E3 ubiquitin transferase PRP19; Senescence evasion factor

Gene Aliases: AA617263; AL024362; D19Wsu55e; hPSO4; NMP200; PRP19; PRPF19; PSO4; SNEV; UBOX4

UniProt ID: (Human) Q9UMS4, (Mouse) Q99KP6, (Rat) Q9JMJ4

Entrez Gene ID: (Human) 27339, (Mouse) 28000, (Rat) 246216

Molecular Function: RNA processing factor