|Tested species reactivity||Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 48 and 328 of PSAT1 (Uniprot ID#Q9Y617)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol, 1% BSA|
|Contains||0.025% Proclin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
PA5-22124 targets PSAT1 in ICC, IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples.
The PA5-22124 immunogen is a recombinant fragment corresponding to a region within amino acids 48 and 328 of PSAT1 (Uniprot ID#Q9Y617).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
NRF2 regulates serine biosynthesis in non-small cell lung cancer.
PA5-22124 was used in western blot to characterize serine biosynthesis and NRF2 regulation in non-small cell lung cancer
|DeNicola GM,Chen PH,Mullarky E,Sudderth JA,Hu Z,Wu D,Tang H,Xie Y,Asara JM,Huffman KE,Wistuba II,Minna JD,DeBerardinis RJ,Cantley LC||Nature genetics (47:1475)||2015|
Phosphoglycerate dehydrogenase is dispensable for breast tumor maintenance and growth.
PA5-22124 was used in western blot to learn how breast tumor maintenance and growth requires phosphogylcerate dehydrogenase
|Chen J,Chung F,Yang G,Pu M,Gao H,Jiang W,Yin H,Capka V,Kasibhatla S,Laffitte B,Jaeger S,Pagliarini R,Chen Y,Zhou W||Oncotarget (4:2502)||2013|