Immunofluorescence analysis of PSD95 (red) in human motor neurons derived from iPSCs. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.2% Triton X-100 in PBS for 10 minutes, and blocked with 5% donkey serum in PBS for 15 minutes at room temperature. Cells were stained with a PSD95 rabbit monoclonal antibody (Product # 700902) diluted at 1:1000 in 5% donkey serum overnight at 4°C, and then incubated with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 546 conjugate (Product # A11010) at a dilution of 1:1000 for 1 hour at room temperature (green). Note: Data courtesy of Innovators Program.
|Tested species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
700902 has been successfully used in immunofluorescence analysis of PSD95 in human motor neurons derived from iPSCs.
PDS-95 (Postsynaptic density-95) also known as Disks large homolog 4 is a protein that in humans is encoded by the DLG4 gene. This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. PSDs were originally identified as an electron-dense region at the membrane of a postsynaptic neuron. PSD-95 interacts with NMDA receptor subunit and potassium channels and is required for synaptic plasticity associated with NMDA receptor signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.