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PSD93 Antibody (701999) in ICC/IF
Immunofluorescence analysis was performed on fixed and permeabilized SH-SY5Y cells for detection of endogenous Chapsyn-110 using Anti-Chapsyn-110 Recombinant Rabbit Monoclonal Antibody (Product # 701999, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Chapsyn-110 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountan... View More
Product Details
701999
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
7H7L3
Immunogen
Protein corresponding to Human DLG2 (aa 250-430)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2633047
Product Specific Information
This antibody is predicted to react with Rat, Mouse, Monkey and Chicken.^M
^M
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
PSD-93, also known as chapsyn-110, is one of a family of plasma membrane-associated proteins found in synaptic junctions. PSD-93 is unique among family members in its expression in Purkinje neuron cell bodies and dendrites. PSD-93 has three ~90 amino acid repeats called PDZ domains, a single interior SH3 domain, and a carboxyl-terminal guanylate kinase homology (GuK) domain that is enzymatically inactive. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticiyt. PSD-93 is believed to participate in the clustering of certain proteins, including NMDA receptors and shaker-type potassium channels at the synaptic membrane. There are two principal modes of interaction between PSD-93 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif may interact with PSD-93 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD-93 in vitro through a pseudo-homotypic PDZ-PDZ interaction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Channel-associated protein of synapse-110; channel-associated protein of synapses, 110kDa; Chapsyn-1; Chapsyn-110; discs large 2; discs large homolog 2; discs, large homolog 2; discs, large homolog 2, chapsyn-110; Disks large homolog 2; Postsynaptic density protein PSD-93; protein phosphatase 1, regulatory subunit 58; synaptic density protein PSD-93
Gene Aliases: A330103J02Rik; B230218P12Rik; B330007M19Rik; chapsyn-110; DLG2; Dlgh2; Gm1197; PPP1R58; PSD-93; PSD93
UniProt ID: (Human) Q15700, (Mouse) Q91XM9, (Rat) Q63622
Entrez Gene ID: (Human) 1740, (Mouse) 23859, (Rat) 64053
chemical synaptic transmission
nervous system development
negative regulation of phosphatase activity
sensory perception of pain
receptor clustering
establishment or maintenance of epithelial cell apical/basal polarity
GMP metabolic process
GDP metabolic process
receptor localization to synapse
neuronal ion channel clustering
nucleotide phosphorylation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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