MA1-753 detects presenilin 2 protein (PS2) from rat, mouse, human, and nonhuman primate samples. No cross-reactivity is seen with presenilin 1.
MA1-753 has successfully been used in immuno-fluorescence, Western blot and ELISA procedures. By Western blot, this antibody detects an ~28 kDa protein representing PS2 NT (N-terminus fragment) and the ~46 kDa full-length PS2 from transfected COS-7 cells. In 4% paraformaldehyde fixed COS-7 cells transfected with hPS2, MA1-753 showed specific Golgi, nuclear envelope, and ER labeling.
The MA1-753 immunogen is a synthetic peptide corresponding to residues C(31) Q E G R Q G P E D G E N T A(45) of human PS2.
Familial Alzheimer's disease is often characterized by an early (<60 years of age) and rapid deterioration of the central nervous system. The symptoms are caused by the abnormal buildup of senile plaques composed of the 42 residue amyloid-beta peptide. This peptide is the result of the amyloid precursor protein being cleaved by the presenilin-gamma-secretase complex. Recent studies have linked mutations in presenilin 1 (PS1) and presenilin 2 (PS2) to a rapid increase in plaque accumulation.
PS1 and PS2 are integral membrane proteins that contain 6-8 transmembrane domains and are predominantly localized within the endoplasmic reticulum and Golgi of neurons within the brain. Within the transmembrane regions of PS1 and PS2 there is over 60% homology, with the largest divergence found at the N-terminus and large loop region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AD3LP; AD4; AD5; Alzheimer disease 4; E5-1; Presenilin-2; Presenilin-2 CTF subunit; Presenilin-2 NTF subunit; PS-2; PS2; PSEN 2; STM-2; STM2
Gene Aliases: AD3L; AD4; Ad4h; ALG-3; Alg3; CMD1V; PS-2; PS2; PSEN2; PSNL2; STM2