Immunofluorescent analysis of PSME3 in HeLa cells using a PSME3 recombinant rabbit monoclonal antibody (Product # 700180) at a dilution of 5ug/ml in the absence of peptide (top) and presence of immunogenic peptide (bottom), followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000. Actin was stained with Alexa Fluor 568 Phalloidin (Product # A12380). Hoechst only (blue, left), AF488 signal only (green, middle) and composite image with Phalloidin (right).
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 7-20 of P61289.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||2-3 ug/test|
|Immunocytochemistry (ICC)||4-6 ug/ml|
|Immunofluorescence (IF)||4-6 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 ug/ml|
|Western Blot (WB)||1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody is predicted to react with canine, chicken, equine, orangutan, Xenopus and zebrafish based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
PA28 alpha and PA28 beta are located primarily in the cytoplasm, whereas PA28 gamma is located in the nucleus. PA28 binds to the outer rings on both ends of the 20S proteasome to form a football-like structure. Binding of PA28 greatly stimulates multiple peptidase activities of the 20S proteasome in an ATP-independent reaction, but lacks the ability to degrade large protein substrates, suggesting that PA28 may cooperate with the 26 S proteasome in a sequential proteolytic pathway. In addition to its nuclear localization, PA28 gamma differs from PA28 alpha and PA28 beta in that it is not responsive to stimulation with IFN- gamma. Experiments with mice lacking the PA28 gamma gene core protein, which targets HCV for degradation, and with MEKK3, which phosphorylates PA28 gamma and increases its cellular levels. PA28 gamma also acts to increase resistance to apoptosis by promoting MDM2-mediated degradation of p53.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mutant p53 (p53-R248Q) functions as an oncogene in promoting endometrial cancer by up-regulating REG¿.
700180 was used in immunohistochemistry and western blot to demonstrate that the p53-R248Q mutant targets the proteasome activator REGgamma to promote endometrial cancer progression
|Wang H,Bao W,Jiang F,Che Q,Chen Z,Wang F,Tong H,Dai C,He X,Liao Y,Liu B,Sun J,Wan X||Cancer letters (360:269)||2015|