|ELISA (ELISA)||0.1-1 µg/mL|
|Immunofluorescence (IF)||0.5 µg/ml|
|Western Blot (WB)||1-3 µg/mL|
|Immunocytochemistry (ICC)||See 1 publications below|
|Immunofluorescence (IF)||See 1 publications below|
|Immunohistochemistry (IHC)||See 3 publications below|
|Western Blot (WB)||See 1 publications below|
|Species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant protein derived from the PTEN protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
32-5800 reacts specifically with the human PTEN protein. Reactivity was confirmed with cell lines which contain endogenous PTEN (NIH3T3, K652, and DU145 prostate cancer line) as well as transfected cell lines (Rat1 and 293T cells). This antibody detects the ~55 kDa PTEN protein and was successfully used in western blotting analysis of PTEN in breast cancer cell lines. No cross-reactivity to any other proteins has been detected.
This gene was identified as a tumor suppressor that is mutated in a variety of tumor cell lines and cancer cell types at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase, PTEN (also known as MMAC1). It contains a tensin-like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate (Ptd-Ins(3,4,5)P3) in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. Ptd-Ins(3,4,5)P3 is a critical second messenger involved in cell signaling and growth control. Ptd-Ins(3,4,5)P3 is produced by phosphorylation of Ptd-Ins(4,5)P2 by PI 3-kinase in response to stimulation by various ligands. By dephosphorylating Ptd-Ins(3,4,5)P3, PTEN functions to ""turn off"" the Ptd-Ins(3,4,5)P3 pathway and thereby suppress cellular proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 4; 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; mitochondrial phosphatase and tensin protein alpha; mitochondrial PTENalpha; MMAC1; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog; phosphatase and tensin-like protein; Phosphatidylinositol 3; Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; TEP1
Gene Aliases: 10q23del; 2310035O07Rik; A130070J02Rik; AI463227; B430203M17Rik; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN; PTEN1; TEP1
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