Molecular weight marker (lane 1). PA5-19417 staining of HeLa whole cell lysate at a dilution of 1 ug/ml (Lane 2). PA5-19417 staining of HEK293 whole cell lysate at a dilution of 1 ug/ml (lane 3). PA5-19417 staining of U2OS whole cell lysate at a dilution of 1 ug/ml (lane 4). Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:15000.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to Human PTIP aa 200-300|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-19417 targets PTIP in WB applications and shows reactivity with Human samples.
The PA5-19417 immunogen is synthetic peptide derived from within residues 150 - 250 of Human PTIP.
PA5-19417 detects PTIP which has a predicted molecular weight of approximately 90 kDa and 100 kDa.
This gene is a member of the paired box gene family and encodes a nuclear protein with six BRCT domains. This protein plays a critical role in maintaining genome stability, condensation of chromatin and progression through mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.