Invitrogen

PTP1B Recombinant Rabbit Monoclonal Antibody (JJ0935)

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Datasheet

Protocols

Questions & Answers

Tech Support

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PTP1B Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of PTPN1 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with PTPN1 Monoclonal Antibody (Product # MA5-32488) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody (Product # A28177) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with DAPI. F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing ER localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

PTP1B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of PTP1B of paraffin-embedded Human tonsil tissue using a PTP1B Monoclonal antibody (Product #MA5-32488). Counter stained with hematoxylin.

PTP1B Antibody in Western Blot (WB) — Western blot was performed using Anti-PTPN1 Monoclonal Antibody (Product # MA5-32488) and a ~ 55kDa band corresponding to PTPN1 was observed in HeLa and Raji cells. An ~45 kDa band corresponding to isoform of PTPN1 was observed across the cell lines tested. An uncharacterized band (*) of ~ 34kDa was also observed in few of the cell lines tested. Membrane enriched extracts (30 µg lysate) of HeLa (Lane 1), Jurkat (Lane 2) HCT 116 (Lane 3), Raji (Lane 4) and MCF-7 (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

PTP1B Antibody in Flow Cytometry (Flow) — Flow Cytometric analysis of PTP1B in Raji cells using a PTP1B Monoclonal Antibody (Product # MA5-32488) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Product Details

MA5-32488

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

Immunohistochemistry (IHC)

1:50-1:200

Immunocytochemistry (ICC/IF)

1:100-1:500

Flow Cytometry (Flow)

1:50-1:100

Product Specifications

Species Reactivity

Human, Zebrafish

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JJ0935

Immunogen

Synthetic peptide within Human PTP1B aa 374-400

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2809765

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

PTP1B (PTPN1) is a tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. It mediates dephosphorylation of EIF2AK3/PERK and in turn inactivates the protein kinase activity of EIF2AK3/PERK. PTP1B also plays a role in CKII- and p60c-src-induced signal transduction cascades. It regulates the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. PTP1B also regulates the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EC 3.1.3.48; hgnc9642; KIAA0283; protein tyrosine phosphatase 1b; protein tyrosine phosphatase, placental; Protein-tyrosine phosphatase 1B; PTN1; PTP-1B; RP5-1121H13.2; RPTPrho; tyrosine phosphatase 1b; tyrosine-phosphatase; Tyrosine-protein phosphatase non-receptor type 1

Gene Aliases: PTP1B; PTPN1; wu:fk54h03

UniProt ID: (Human) P18031

Entrez Gene ID: (Human) 5770, (Zebrafish) 30081

Molecular Function: protein phosphatase

Function(s)

protein dephosphorylation activation of signaling protein activity involved in unfolded protein response activation of JUN kinase activity insulin receptor signaling pathway regulation of signal transduction negative regulation of signal transduction regulation of endocytosis negative regulation of vascular endothelial growth factor receptor signaling pathway endoplasmic reticulum unfolded protein response actin cytoskeleton reorganization regulation of intracellular protein transport peptidyl-tyrosine dephosphorylation platelet-derived growth factor receptor-beta signaling pathway IRE1-mediated unfolded protein response negative regulation of MAP kinase activity negative regulation of insulin receptor signaling pathway regulation of type I interferon-mediated signaling pathway JAK-STAT cascade involved in growth hormone signaling pathway positive regulation of protein tyrosine kinase activity negative regulation of ERK1 and ERK2 cascade cell-cell adhesion regulation of hepatocyte growth factor receptor signaling pathway negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of IRE1-mediated unfolded protein response negative regulation of PERK-mediated unfolded protein response peptidyl-tyrosine dephosphorylation involved in inactivation of protein kinase activity positive regulation of receptor catabolic process dephosphorylation

Process(es)

protein tyrosine phosphatase activity insulin receptor binding protein binding zinc ion binding enzyme binding protein kinase binding receptor tyrosine kinase binding poly(A) RNA binding ephrin receptor binding protein phosphatase 2A binding cadherin binding involved in cell-cell adhesion phosphoprotein phosphatase activity hydrolase activity phosphatase activity