|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human protein purified from E.coli|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is Bosc23 cells transfected with Myc/His/HePTP.
Hematopoietic tyrosine phosphatase (HePTP, PTPN7) belongs to a subgroup of PTPases with two other members, STEP and PCPTP1. HePTP is a 38 kDa class I non-receptor protein tyrosine phosphatase (PTP) that is strongly expressed in T cells. The protein consists of a short, amino-terminal extension that contains a conserved 16-amino acid kinase interaction motif (KIM) and a carboxy-terminal protein tyrosine phosphatase domain. HePTP binds the MAPK family kinases, Erk1/2 and p38 via KIM. Erk phosphorylates HePTP (at Thr45 and Ser72) and HePTP dephosphorylates Erk (at Tyr185). HePTP is phosphorylated at Ser23 within the KIM by PKA in T cells. This phosphorylation results in dissociation of the HePTP/ERK2 complex and reduced ability of HePTP to dephosphorylate ERK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.