Western blot analysis of PTPRR using anti-PTPRR monoclonal antibody (MA5-17254) on (Lane 1) Bosc23 Cell lysate, (Lane 2) Bosc23 Cell lysate transfected with Myc/His/Tau, (Lane 3) Bosc23 Cell lysateMyc/His/mRNA-capping enzyme and (Lane 4) Bosc23 Cell lysate GFP-Ub-1.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant human protein purified from E.coli|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 ug|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is Bosc23 cell lysate transfected with Myc/His/PTPRR.
Sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. The MAPKs bind to a dephosphorylated kinase interacting motif, phosphorylation of which by the protein kinase A complex releases the MAPKs for activation and translocation into the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.