|Tested species reactivity||Human, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant protein corresponding to amino acids 76 - 170 of human PUMA-alpha.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||2.5-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control is K562 cell lysate.
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.