|Tested species reactivity||Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (PLPRGHRAPEMEPN) corresponding to amino acids 180 to 193 of human PUMA-a|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
PA1-30829 detects PUMA from mouse, human samples.
PA1-30829 has been successfully used in Western blot applications. K562 or 3T3 cell lysate can be used as a positive control and a band at approximately 23 kDa can be detected. A lower band at approximately 16 kDa was detected in MOLT4 and U937 cells, which may represent the PUMA-beta form.
The PA1-30829 immunogen is synthetic peptide (PLPRGHRAPEMEPN) corresponding to amino acids 180 to 193 of human PUMA-a (1).
A novel gene PUMA (p53 upregulated modulator of apoptosis) is a target for activation by the p53 tumor-suppressor gene. p53 functions as a transcriptional activator, and influences p53-inducible genes that play a role in the induction of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Caspase-3 triggers a TPCK-sensitive protease pathway leading to degradation of the BH3-only protein puma.
PA1-30829 was used in immunoprecipitation and western blot to investigate the effect of caspase-3 on puma degradation
|Hadji A,Clybouw C,Auffredou MT,Alexia C,Poalas K,Burlion A,Feraud O,Leca G,Vazquez A||Apoptosis : an international journal on programmed cell death (15:1529)||2010|