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|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 36-53 of PUMA-delta and 196-213 of PUMA-gamma.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||2-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Some cross reactivity is seen with PUMA alpha.
This sequence is 59% homologus to PUMA alpha.
Suggested positive control: HeLa, NIH-3T3 cell lysate.
A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) in human and mouse. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. PUMA-g is a novel member of the seven transmembrane spanning superfamily.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bcl-2 binding component 3; bcl-2-binding component 3; BH3-only; FLJ42994; JFY1; p53 up-regulated modulator of apoptosis; PUMA
BBC3; JFY-1; JFY1; PUMA; PUMA/JFY1