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OriGene
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:2,000 | - |
Immunocytochemistry (ICC/IF) |
1:100 | - |
Flow Cytometry (Flow) |
1:100 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Dog, Human, Rat |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
OTI4C6 |
Immunogen |
Full length human recombinant protein of human PUS7 produced in HEK293T cell. |
Conjugate |
Unconjugated |
Form |
lyophilized |
Concentration |
1 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 8% trehalose |
Contains |
no preservative |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Pseudouridylate synthase that catalyzes pseudouridylation of RNAs (PubMed:28073919, PubMed:29628141, PubMed:30778726). Acts as a regulator of protein synthesis in embryonic stem cells by mediating pseudouridylation of RNA fragments derived from tRNAs (tRFs): pseudouridylated tRFs inhibit translation by targeting the translation initiation complex (PubMed:29628141). Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3' (PubMed:28073919). In addition to mRNAs and tRNAs, binds other types of RNAs, such as snRNAs, Y RNAs and vault RNAs, suggesting that it can catalyze pseudouridylation of many RNA types (PubMed:29628141). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: FLJ20485; pseudouridylate synthase 7 (putative); Pseudouridylate synthase 7 homolog
Gene Aliases: KIAA1897; PUS7; RGD1307054
UniProt ID: (Human) Q96PZ0
Entrez Gene ID: (Human) 54517, (Dog) 100856353, (Rat) 296751
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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