Invitrogen

PYGO2 Monoclonal Antibody (539730)

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PYGO2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of PYGO2 in immersion fixed MCF‚7 human breast cancer cell line. Samples were incubated in PYGO2 monoclonal antibody (Product # MA5-24240) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.

PYGO2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of PYGO2 in immersion fixed paraffin-embedded sections of normal human ovary (left panel) and ovarian cancer tissue (right panel). Samples were incubated in PYGO2 monoclonal antibody (Product # MA5-24240) using a dilution of 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei.

PYGO2 Antibody in Western Blot (WB) — Western blot analysis of PYGO2 in HeLa human cervical epithelial carcinoma cell line, MCF‚7 human breast cancer cell line, and Daudi human Burkitts lymphoma cell line. Samples were incubated in PYGO2 monoclonal antibody (Product # MA5-24240) using a dilution of 0.5 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for Pygopus‚2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions.

PYGO2 Antibody in Western Blot (WB) — Western blot analysis of PYGO2 in 0.2 mg/mL lysates of HeLa human cervical epithelial carcinoma cell line. Samples were incubated in PYGO2 monoclonal antibody (Product # MA5-24240) using a dilution of 5 µg/mL. A specific band was detected for Pygopus‚2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Product Details

MA5-24240

Applications	Tested Dilution	Publications
Western Blot (WB)	0.5-5 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	3-25 µg/mL	-
Immunocytochemistry (ICC/IF)	8-25 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Clone	539730
Immunogen	E. coli-derived recombinant human Pygopus-2 Met160-Glu336 View antigen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	0.5 mg/mL
Purification	Protein A/G
Storage buffer	PBS with 5% trehalose
Contains	No Preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2605750

Product Specific Information

In direct ELISAs, no cross-reactivity with recombinant human Pygopus-1 or recombinant mouse Pygopus-1 is observed.

Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.

Target Information

Involved in signal transduction through the Wnt pathway. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: mpygo2; pygopus 2; Pygopus homolog 2

Gene Aliases: 1190004M21Rik; PP7910; PYGO2

UniProt ID: (Human) Q9BRQ0

Entrez Gene ID: (Human) 90780

in utero embryonic development kidney development lens development in camera-type eye spermatid nucleus differentiation brain development post-embryonic development mammary gland development regulation of mammary gland epithelial cell proliferation regulation of histone acetylation positive regulation of chromatin binding developmental growth regulation of histone H3-K4 methylation palate development canonical Wnt signaling pathway beta-catenin-TCF complex assembly

Process(es)

chromatin binding protein binding zinc ion binding histone acetyltransferase regulator activity histone binding