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PYGO2 Antibody (MA5-24240) in ICC/IF
Immunocytochemistry analysis of PYGO2 in immersion fixed MCF‚7 human breast cancer cell line. Samples were incubated in PYGO2 monoclonal antibody (Product # MA5-24240) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.
Product Details
MA5-24240
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
539730
Immunogen
E. coli-derived recombinant human Pygopus-2 Met160-Glu336
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2605750
Product Specific Information
In direct ELISAs, no cross-reactivity with recombinant human Pygopus-1 or recombinant mouse Pygopus-1 is observed.
Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
Target Information
The PYGO2 gene encodes pygopus family PHD finger 2, a key component of the Wnt signaling pathway, which is critical for embryonic development, cell proliferation, and differentiation. PYGO2 operates as a chromatin remodeling factor, interacting with the beta-catenin-LEF/TCF transcriptional complex, facilitating the transcription of Wnt target genes by recruiting chromatin-modifying enzymes. This activity is vital for maintaining the balance between stem cell renewal and differentiation in various tissues, including the brain and the skin. Additionally, PYGO2 plays significant roles in spermatogenesis and hematopoiesis by modulating gene expression necessary for these processes. Dysregulation of PYGO2 expression can lead to developmental abnormalities and is often implicated in cancer progression, indicating its potential as a biomarker for tumorigenic stem cell-like properties and metastatic behavior. Research continues to explore PYGO2's mechanisms and interactions to understand its contributions to development and its implications in disease states, particularly in cancer biology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: mpygo2; pygopus 2; Pygopus homolog 2
Gene Aliases: 1190004M21Rik; PP7910; PYGO2
UniProt ID: (Human) Q9BRQ0
Entrez Gene ID: (Human) 90780
in utero embryonic development
kidney development
lens development in camera-type eye
spermatid nucleus differentiation
brain development
post-embryonic development
mammary gland development
regulation of mammary gland epithelial cell proliferation
regulation of histone acetylation
positive regulation of chromatin binding
developmental growth
regulation of histone H3-K4 methylation
palate development
canonical Wnt signaling pathway
beta-catenin-TCF complex assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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