|Tested species reactivity||Chicken, Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Endogenous Palladin immunoprecipitated from chicken embryo fibroblasts.|
|Storage buffer||tris glycine with 0.15M NaCl|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Immunoprecipitation (IP)||0.05 ml/2 ml lysate|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: MDA-MB231 (human breast cancer) lysates.
Palladin is a cytoskeletal protein required for organization of normal actin cytoskeleton. Palladin plays roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. Palladin may function as a scaffolding molecule with the potential to impact both actin polymerization as well as the assembly of existing actin filaments into higher-order arrays. It binds to proteins that bind to either monomeric or filamentous actin. It localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Palladin's different isoforms may have functional differences. It is also involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.