|Tested species reactivity||Bovine, Fruit fly, Human, Mouse, Non-human primate, Rat, Zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Two synthetic peptides corresponding to two conserved regions of human N-Cadherin|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
IP-MS enrichment using a Cadherin pan antibody (LFQ intensity): CDH1 was enriched 36-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and Cadherin pan antibody (Part No. PA5-17526). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
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