Description: The monoclonal antibodies AE1 and AE3 recognize many of the acidic and basic cytokeratin family members. Cytokeratins are intermediate filament proteins comprising one component of the cytoskeleton. There are two large families of cytokeratins, acidic and basic, but all contain the same basic domains (i.e. an alpha-helical core with an N- and C-terminal domain). The proteins are expressed in epithelial cells, but are developmentally regulated. Many tumors also express these proteins and their expression can help identify the origin of a neoplasm.
The AE3 monoclonal antibody recognizes the 65 to 67 triplet, 64, 59, 58, 56, 54 and 52kD proteins also known as cytokeratin 1, 2, 3, 4, 5, 6, 7, and 8 while the AE1 antibody recognizes 56.5, 54', 50, 50', 48, and 40 kDa proteins (also known as CK10, 14, 15, 16 and 19). These antibodies can be used on a wide array of tissue samples from mouse, human, rat, primates (cynomolgus and rhesus), dog, cat, rabbit, and chicken.
Applications Reported: This AE1/AE3 antibody has been reported for use in immunohistochemical staining, immunocytochemistry, immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.
Applications Tested: This AE1/AE3 antibody has been tested by immunocytochemistry on methanol-fixed MCF7 cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 40-kDa keratin intermediate filament; 47 kDa cytokeratin; 56 kDa cytokeratin; 57kd keratin; 57kDa keratin; 58 kDa cytokeratin; 60-kDa keratin; 65 kDa cytokeratin; 67 kDa cytokeratin; Cell proliferation-inducing gene 46 protein; CK-1; CK-10; CK-13; CK-14; CK-15; CK-16; CK-18; CK-19; CK-2e; CK-3; CK-4; CK-5; CK-6A; CK-6D; CK-7; CK-8; CK5; cytokeratin 1; cytokeratin 10; cytokeratin 13; cytokeratin 14; cytokeratin 15; cytokeratin 16; cytokeratin 18; cytokeratin 19; cytokeratin 3; cytokeratin 4; cytokeratin 5; cytokeratin 6A; cytokeratin 6C; cytokeratin 6D; Cytokeratin 7; cytokeratin 8; Cytokeratin endo A; Cytokeratin endo B; Cytokeratin-1; Cytokeratin-10; Cytokeratin-13; Cytokeratin-14; Cytokeratin-15; Cytokeratin-16; Cytokeratin-18; Cytokeratin-19; Cytokeratin-2e; Cytokeratin-3; Cytokeratin-4; Cytokeratin-5; Cytokeratin-6A; cytokeratin-6C; Cytokeratin-6D; cytokeratin-6E; Cytokeratin-7; Cytokeratin-8; Cytoskeletal 57 kDa keratin; DDD; EBS2; Endo B; epidermal keratin 10; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; epidermolysis bullosa simplex, Dowling-Meara, Koebner; epidermolytic hyperkeratosis 1; epithelial keratin 1; epithelial keratin 10; epithelial keratin 2e; Epithelial keratin-1; Epithelial keratin-2e; focal non-epidermolytic palmoplantar keratoderma; Hair alpha protein; Hom s 5; intermediate filament protein; K1; K10; K13; K14; K15; K16; K18; K19; K2e; K3; K3 keratin; K4; K5; K6A; K7; K8; kamp-keratin derived antimicrobial peptide; KDAMP; keratin 1; keratin 1 (epidermolytic hyperkeratosis); keratin 1, type II; keratin 10; keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris); keratin 10, type I; keratin 13; keratin 13, type I; keratin 13-like; keratin 14; keratin 14, type I; keratin 14-like; keratin 15; keratin 15, type I; keratin 16 (focal non-epidermolytic palmoplantar keratoderma); keratin 16, type I; keratin 18; keratin 18, type I; keratin 18-like; keratin 19; keratin 19, type I; keratin 2; keratin 2 epidermis; keratin 2, type II; keratin 2A (epidermal ichthyosis bullosa of Siemens); keratin 3; keratin 3, type II; keratin 4; keratin 4, type II; keratin 5; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin 5-like; keratin 6A; keratin 6A, , type II; keratin 6A, type II; keratin 6A-like; keratin 6B; keratin 7; keratin 7, type II; keratin 8; keratin 8, type II; keratin 86; keratin complex 1, acidic, gene 10; keratin complex 1, acidic, gene 13; keratin complex 1, acidic, gene 14; keratin complex 1, acidic, gene 15; keratin complex 1, acidic, gene 16; keratin complex 1, acidic, gene 18; keratin complex 1, acidic, gene 19; keratin complex 2, basic, gene 1; keratin complex 2, basic, gene 17; keratin complex 2, basic, gene 4; keratin complex 2, basic, gene 5; keratin complex 2, basic, gene 6a; keratin complex 2, basic, gene 6c; keratin complex 2, basic, gene 7; keratin complex 2, basic, gene 8; keratin complex 2, gene 17; keratin complex 2, gene 6a; Keratin D; keratin intermediate filament 16a; keratin intermediate filament 16b; keratin K3; keratin K6h; keratin, 55K type II cytoskeletal; keratin, epidermal type II, K6A; keratin, simple epithelial type I, K7; Keratin, type I cytoskeletal 10; keratin, type I cytoskeletal 10-like; Keratin, type I cytoskeletal 13; Keratin, type I cytoskeletal 14; Keratin, type I cytoskeletal 15; Keratin, type I cytoskeletal 16; Keratin, type I cytoskeletal 18; Keratin, type I cytoskeletal 19; Keratin, type I cytoskeletal 59 kDa; keratin, type I, 40-kd; Keratin, type II cytoskeletal 1; Keratin, type II cytoskeletal 2 epidermal; Keratin, type II cytoskeletal 3; keratin, type II cytoskeletal 3-like; Keratin, type II cytoskeletal 4; Keratin, type II cytoskeletal 5; keratin, type II cytoskeletal 5-like; Keratin, type II cytoskeletal 6A; Keratin, type II cytoskeletal 7; Keratin, type II cytoskeletal 8; Keratin-1; Keratin-10; Keratin-13; Keratin-14; Keratin-15; keratin-15, basic; keratin-15, beta; Keratin-16; Keratin-18; Keratin-19; Keratin-2 epidermis; Keratin-2e; Keratin-3; Keratin-4; Keratin-5; Keratin-6-alpha; Keratin-6A; keratin-6C; Keratin-7; Keratin-8; KRT1; krt2; krt5; KRT5A; krt7; mK6-alpha; Sarcolectin; suprabasal cytokeratin 10; tuftelin interacting protein 8; type I cytoskeletal 15; type II mesothelial keratin K7; Type-II keratin Kb1; type-II keratin Kb12; Type-II keratin Kb2; Type-II keratin Kb3; Type-II keratin Kb4; Type-II keratin Kb5; Type-II keratin Kb6; Type-II keratin Kb7; Type-II keratin Kb8; uncharacterized LOC706830; uncharacterized LOC707236
Gene Aliases: 2310016L08Rik; 3300001P10Rik; AA960620; AI324768; AI528832; AI626930; AI663979; AL022697; AU019895; AW108092; AW146334; BB005427; BCIE; BIE; CARD2; CK-10; CK-18; CK-2e; CK-3; CK-4; CK-6C; CK-6E; CK-8; CK1; CK10; CK13; CK14; CK15; CK16; CK18; CK19; CK3; CK4; CK5; CK6A; CK6C; CK6D; CK7; CK8; CYK18; CYK4; CYK8; D130054E02Rik; D15Wsu77e; DDD; DDD1; EBS2; EBS3; EBS4; EGK_03684; EGK_03685; EHK; EHK1; EndoA; EndoC; EPPK; FNEPPK; K1; K10; K13; K14; K15; K16; K18; K19; K1C1; K1CO; K1CP; K1CS; K2C7; K2C8; K2E; K3; K4; K5; K6A; K6C; K6D; K7; K8; KER1; Ker10; Ker2; Kerd; KO; KPP; Krt-1.10; Krt-1.13; Krt-1.14; Krt-1.19; Krt-2.1; Krt-2.4; Krt-2.8; KRT1; Krt1-10; Krt1-13; Krt1-14; Krt1-15; Krt1-16; Krt1-18; Krt1-19; KRT10; KRT13; KRT14; KRT15; KRT16; KRT16A; KRT18; KRT19; KRT1A; KRT2; Krt2-1; Krt2-17; Krt2-2; Krt2-4; Krt2-5; Krt2-6; Krt2-6a; Krt2-6c; Krt2-7; Krt2-8; KRT2A; KRT2E; KRT3; Krt4; KRT5; KRT5A; Krt6; KRT6A; KRT6B; KRT6C; KRT6D; KRT7; KRT8; Krt86; KRTA; KRTB; KRTE; mK6[a]; MK6a; NEPPK; NFJ; PC1; PC3; PIG46; SCL; Tfip8; WSN1; WSN2
UniProt ID: (Dog) Q6EIY9, (Human) P04264, (Human) P13645, (Human) P13646, (Human) P02533, (Human) P19012, (Human) P08779, (Human) P05783, (Human) P08727, (Human) P35908, (Human) P12035, (Rabbit) Q29426, (Human) P13647, (Human) P02538, (Human) P08729, (Human) P05787, (Mouse) P04104, (Mouse) P02535, (Mouse) P08730, (Mouse) Q61781, (Mouse) Q61414, (Mouse) Q9Z2K1, (Mouse) P05784, (Mouse) P19001, (Mouse) Q3TTY5, (Mouse) P07744, (Mouse) Q922U2, (Mouse) P50446, (Mouse) Q9DCV7, (Mouse) P11679
Entrez Gene ID: (Dog) 444857, (Rhesus monkey) 699320, (Human) 3848, (Rabbit) 100009029, (Dog) 491006, (Human) 3858, (Human) 3860, (Rhesus monkey) 100426180, (Human) 3861, (Dog) 100685323, (Dog) 100685404, (Human) 3866, (Dog) 490978, (Human) 3868, (Human) 3875, (Dog) 477601, (Human) 3880, (Dog) 100685481, (Rhesus monkey) 699567, (Human) 3849, (Dog) 444850, (Human) 3850, (Dog) 100683401, (Rabbit) 100353668, (Human) 3851, (Dog) 100682832, (Rhesus monkey) 700060, (Human) 3852, (Dog) 100687760, (Human) 3853, (Dog) 477602, (Human) 3855, (Rhesus monkey) 697425, (Rabbit) 100348891, (Human) 3856, (Dog) 486513, (Rhesus monkey) 700449, (Mouse) 16678, (Mouse) 16661, (Mouse) 16663, (Mouse) 16664, (Mouse) 16665, (Mouse) 16666, (Mouse) 16668, (Mouse) 16669, (Mouse) 16681, (Mouse) 16682, (Mouse) 110308, (Mouse) 16687, (Mouse) 110310, (Mouse) 16691, (Rabbit) 100341120, (Rabbit) 100341376, (Rabbit) 100352658, (Rabbit) 100353415, (Rabbit) 100354174, (Rhesus monkey) 100424400, (Rhesus monkey) 100425096, (Dog) 486523, (Dog) 490983, (Rhesus monkey) 702153, (Rhesus monkey) 706830, (Rhesus monkey) 707236