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OriGene
Pancreatic Polypeptide (PPY) Monoclonal Antibody (OTI1G7)
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FIGURE: 1 / 1
Pancreatic Polypeptide (PPY) Antibody (CF813059) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPY (RC206584, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPY (1:500).
Product Details
CF813059
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1G7
Immunogen
Human recombinant protein fragment corresponding to amino acids 30-88 of human PPY (NP_002713) produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 8% trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
The PPY gene encodes pancreatic polypeptide, a peptide hormone involved in the regulation of appetite and energy balance. The origin of the PPY gene is linked to a tandem duplication of the PYY gene, occurring concomitantly with the emergence of tetrapods, leading to the distinct PPY function within the neuropeptide Y family. In primates and ungulates, a gene duplication event resulted in the formation of a PYY2-PPY2 gene cluster, highlighting the evolutionary complexity of these genes. The PPY gene product is secreted by gamma-cells in the pancreas, localized primarily in the islet periphery, and plays a crucial role in metabolic homeostasis by interacting with receptors in the central nervous system to modulate satiety signals postprandially. PPY's function is linked to its inhibition of AGRP/NPY neurons which decreases their suppressive effect on POMC neurons, enhancing POMC activity and contributing to appetite suppression. This intricate regulation underscores the importance of PPY in dietary intake and metabolic disorders, with ongoing research exploring its potential therapeutic applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HGNC:9327; IOPPP; Pancreatic polypeptide prohormone; Pancreatic polypeptide Y; PH; PP1; PPA1; PPase; prepro-PP (prepropancreatic polypeptide); SID6-8061
Gene Aliases: PNP; PP; PPY
UniProt ID: (Human) P01298
Entrez Gene ID: (Human) 5539
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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