Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunofluorescent analysis of Pannexin-1 in HEK293 cells using a Pannexin-1 recombinant rabbit oligoclonal antibody (Product # 710184) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). b: Image of DAPI stained nucleus (blue) and Alexa Fluor 594 phalloidin stained actin (red) c: Composite image of cells showing cell-membrane and junction localization of Pannexin 1.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to amino acids 347–360 of human pannexin 1|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-3 ug/ml|
|Immunofluorescence (IF)||1-3 ug/ml|
|Western Blot (WB)||1-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Pannexin 1 is a member of a new family of gap junction proteins. It is abundantly expressed in the central nervous system (CNS) and is coexpressed with Pannexin 2 in various neuronal populations. Pannexin 1 can also form a mechanosensitive ion channel that is also ATP-permeable in the nonjunctional plasma membrane. It also plays a role as a Ca2+-leak channel to regulate Ca2+ homeostasis in the endoplasmic reticulum.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
innexin; MRS1; Pannexin 1; PANX1; PX1; UNQ2529; UNQ2529/PRO6028
MRS1; PANX1; PX1; UNQ2529; UNQ2529/PRO6028