Western blot analysis of Pannexin 1 was performed by loading 20 ug of Rat Brian (lane1) and Mouse Brain (lane2) tissue lysate using Novex® NuPAGE® 12 % Bis-Tris gel (NP0322BOX), XCell SureLock™ Electrophoresis System (EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk at 4°C overnight. Pannexin 1 was detected at ~ 45 kDa using Pannexin 1 Rabbit Polyclonal Antibody (488000) at 2-3 ug/ml in 5 % skim milk at 4°C overnight on a rocking platform. Goat Anti-Rabbit IgG - HRP Secondary Antibody (G21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (WP20005).
|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the internal region of the mouse Pannexin 1 protein (Accession# NP_062355), which is identical to rat and human sequence.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||2-4 ug/ml|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
The pannexin gene family encodes a second class of putative gap junction proteins and are highly conserved in invertebrates and mammals. Pannexins (Panx) are four-pass transmembrane proteins that oligomerize to form large pore ion and metabolite-permeable channels. Pannexin-1 (PANX1) and Pannexin-3 are closely related, while Pannexin-2 is a more distant relation. PANX1 is a transmembrane protein that forms a mechanosensitive ATP-permeable channel between adjacent cells and in the endoplasmic reticulum. PANX1 may play a role as a Ca2+ -leak channel to regulate ER Ca2+ homeostasis and regulates neural stem and progenitor cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Pannexin-1 Up-regulation in the Dorsal Root Ganglion Contributes to Neuropathic Pain Development.
488000 was used in western blot to identify the epigenetic mechanism involved in the increased Panx1 expression in the dorsal root ganglion after nerve injury.
|Zhang Y,Laumet G,Chen SR,Hittelman WN,Pan HL||The Journal of biological chemistry (290:14647)||2015|