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Western blot analysis of Pannexin 1 was performed by loading 20 ug of Rat Brian (lane1) and Mouse Brain (lane2) tissue lysate using Novex® NuPAGE® 12 % Bis-Tris gel (NP0322BOX), XCell SureLock™ Electrophoresis System (EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk at 4°C overnight. Pannexin 1 was detected at ~ 45 kDa using Pannexin 1 Rabbit Polyclonal Antibody (488000) at 2-3 ug/ml in 5 % skim milk at 4°C overnight on a rocking platform. Goat Anti-Rabbit IgG - HRP Secondary Antibody (G21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (WP20005).
|Tested species reactivity||Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the internal region of the mouse Pannexin 1 protein (Accession# NP_062355), which is identical to rat and human sequence.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||2-4 ug/ml|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The pannexin gene family encodes a second class of putative gap junction proteins and are highly conserved in invertebrates and mammals. Pannexins (Panx) are four-pass transmembrane proteins that oligomerize to form large pore ion and metabolite-permeable channels. Pannexin-1 (PANX1) and Pannexin-3 are closely related, while Pannexin-2 is a more distant relation. PANX1 is a transmembrane protein that forms a mechanosensitive ATP-permeable channel between adjacent cells and in the endoplasmic reticulum. PANX1 may play a role as a Ca2+ -leak channel to regulate ER Ca2+ homeostasis and regulates neural stem and progenitor cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
MRS1; Pannexin 1; pannexin-1; PANX1; PX1; UNQ2529; UNQ2529/PRO6028