Immunofluorescent analysis of Par-6 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Par-6 polyclonal antibody (Product # PA3-911) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Combination of two synthetic peptides coupled to KLH.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA3-911detects Par-6 from human and mouse samples.
PA3-911has been successfully used in Western blot and ICC/IF procedures. This antibody is not recommended for U87-MG cells in Western blot applications.
The PA3-911 immunogen is a combination of two synthetic peptides coupled to KLH.
Par-6 is a epithelial polarity protein important to a number of developmental processes for migration. Depletion of Par-6 results in disorganization of the border cells cluster and impaired migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.