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Product Details
Z2378MS
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
ZM207
Immunogen
A synthetic peptide around aa 1-34 of human mature-PTH-polypeptide; A recombinant fragment around aa 32-115 of human mature PTH-polypeptide (exact sequence is proprietary)
Conjugate
Form
Liquid
Concentration
200 µg/mL
Purification
Protein A
Storage buffer
tris with NP-40, BSA
Contains
<0.1% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
A recommended positive control tissue for this product is Parathyroid, however positive controls are not limited to this tissue type.
The primary antibody is intended for laboratory professional use in the detection of the corresponding protein in formalin-fixed, paraffin-embedded tissue stained in manual qualitative immunohistochemistry (IHC) testing. This antibody is intended to be used after the primary diagnosis of tumor has been made by conventional histopathology using non-immunological histochemical stains.
PTH is produced in the parathyroid gland as an 84 amino acid single chain polypeptide. It can also be secreted as N-terminal truncated fragments or C-terminal fragments after intracellular degradation, as in case of hypercalcemia. Defects in this gene are a cause of familial isolated hypoparathyroidism (FIH); also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.
Antibody is used with formalin-fixed and paraffin-embedded sections. Pretreatment of deparaffinized tissue with heat-induced epitope retrieval or enzymatic retrieval is recommended. In general, immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody (link antibody), an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.
A positive tissue control must be run with every staining procedure performed. This tissue may contain both positive and negative staining cells or tissue components and serve as both the positive and negative control tissue. External Positive control materials should be fresh autopsy/biopsy/surgical specimens fixed, processed and embedded as soon as possible in the same manner as the patient sample (s). Positive tissue controls are indicative of correctly prepared tissues and proper staining methods. The tissues used for the external positive control materials should be selected from the patient specimens with well-characterized low levels of the positive target activity that gives weak positive staining. The low level of positivity for external positive controls is designed to ensure detection of subtle changes in the primary antibody sensitivity from instability or problems with the staining methodology. A tissue with weak positive staining is more suitable for optimal quality control and for detecting minor levels of reagent degradation.
Internal or external negative control tissue may be used depending on the guidelines and policies that govern the organization to which the end user belongs to. The variety of cell types present in many tissue sections offers internal negative control sites, but this should be verified by the user. The components that do not stain should demonstrate the absence of specific staining, and provide an indication of non-specific background staining. If specific staining occurs in the negative tissue control sites, results with the patient specimens must be considered invalid.
Target Information
This gene encodes a member of the parathyroid family of proteins. The encoded preproprotein is proteolytically processed to generate a protein that binds to the parathyroid hormone/parathyroid hormone-related peptide receptor and regulates blood calcium and phosphate levels. Excess production of the encoded protein, known as hyperparathyroidism, can result in hypercalcemia and kidney stones. On the other hand, defective processing of the encoded protein may lead to hypoparathyroidism, which can result in hypocalcemia and numbness.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Parathormone; Parathyrin; Parathyroid hormone; parathyroid hormone 1; prepro-PTH; preproparathyroid hormone; PTH
Gene Aliases: PTH; PTH1
UniProt ID: (Human) P01270
Entrez Gene ID: (Human) 5741
negative regulation of transcription from RNA polymerase II promoter
skeletal system development
cellular calcium ion homeostasis
G-protein coupled receptor signaling pathway
adenylate cyclase-activating G-protein coupled receptor signaling pathway
Rho protein signal transduction
cell-cell signaling
hormone-mediated apoptotic signaling pathway
positive regulation of signal transduction
response to lead ion
regulation of gene expression
positive regulation of bone mineralization
positive regulation of cAMP biosynthetic process
response to vitamin D
cellular macromolecule biosynthetic process
response to drug
bone resorption
response to ethanol
positive regulation of glycogen biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
cAMP metabolic process
positive regulation of glucose import
response to cadmium ion
homeostasis of number of cells within a tissue
response to parathyroid hormone
response to fibroblast growth factor
positive regulation of cell proliferation in bone marrow
negative regulation of apoptotic process in bone marrow
positive regulation of osteoclast proliferation
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