Immunofluorescent analysis of Paxillin in HeLa cells, fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Polyethylene glycol tert-octylphenyl ether, using a Paxillin monoclonal antibody (Product # MA1-24952) at a dilution of 1:200 followed by detection using a Goat anti-Mouse IgG, Cy3 conjugate.
|Tested species reactivity||Bovine, Chicken, Hamster, Human, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant fragment (residues 305-559) of chicken Paxillin.|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-24952 detects Paxillin from human, rat, bovine, chicken, and hamster samples.
MA1-24952 has been successfully used in Western blot procedures.
The MA1-24952 immunogen is a recombinant fragment (residues 305-559) of chicken Paxillin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.