A suggested positive control is human liver tissue lysate.
PA5-34465 can be used with blocking peptide PEP-1507.
The Pellino proteins are a highly homologous family of E3 ubiquitin ligases that act as upstream mediators in Toll-like receptor (TLR) pathways that lead to activation of MAP kinases and transcription factors. Pellino 1 is required for interleukin-1-mediated signaling through its interaction with the IRAK4-IRAK-TRAF6 complex, ultimately resulting in the activation of NF-Kappa-B. Like other members of the Pellino family, Pellino 1 is an E3 ubiquitin ligase, able to catalyze the polyubiquitination of IRAK1. It is activated via phosphorylation by either IRAK1 and IRAK4 or the IKK-related kinases IKK-epsilon; and TBK1. In addition to phosphorylation, Pellino 1 activity is also modulated via ubiquitination and sumoylation.
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Protein Aliases: E3 ubiquitin-protein ligase pellino homolog 1; PELI1; Pellino 1; Pellino E3 ubiquitin protein ligase 1; pellino homolog 1; Pellino-1; Pellino-related intracellular-signaling molecule; pellino1; protein pellino homolog 1; RING-type E3 ubiquitin transferase pellino homolog 1
Gene Aliases: 2810468L03Rik; A930031K15Rik; AA409794; AI586297; D11Ertd676e; PELI1; PRISM